Journal of Nanomaterials & Molecular NanotechnologyISSN: 2324-8777

Reach Us +44-7480-724769
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Spherical Gold Nanoparticles Impede the Function of Bovine Serum Albumin In vitro: A New Consideration for Studies in Nanotoxicology

Spherical Gold Nanoparticles Impede the Function of Bovine Serum Albumin In vitro: A New

Suspensions of bovine serum albumin (BSA) and spherical gold nanoparticles were analyzed to determine if gold nanoparticles (nAu) affect the ligand binding properties of BSA. A range of diameters of nAu with a carboxylic acid capping agent (nAu-cap) were tested, along with nanoparticles conjugated to amine (nAu-NH3+) and carboxyl (nAu-COO-) functional groups via a covalent polymer bridge. All nAu tested were found to affect BSA conformation as determined by intrinsic tryptophan fluorescence. Smaller diameters of nAu-cap (30-50 nm), along with nAu-NH3+ and nAu-COO-, impeded the binding of 8-anilino-1-napthalenesulfonic acid (ANS) to BSA. Similarly, smaller diameters of nAu-cap tended to impede oleic acid binding to BSA, with a linear negative correlation observed between nAu-cap diameter and the dissociation constant (KD) of oleic acid over the range of 40-80 nm. 80 nm nAu-cap impeded butanoic acid binding, and necessitated a high-resolution fluorescence assay. As with oleic acid, smaller diameters of nAu-cap tended to impede ibuprofen binding, but no significance could be established.

Special Features

Full Text

View

Track Your Manuscript

Share This Page

Media Partners

Associations