Anubha Palchaudhuri Author

Research Article Open Access

Gamma-Delta or Transposon Tn1000 forms Unstable F-Prime Plasmid KLF-5 in the Proximity of Oric

Author(s):

Sunil Palchaudhuri, Anubha Palchaudhuri and Shreya Bhattacharyya

The F prime plasmid KLF5 consists of two components, one component is F plasmid (100Kb) and the other component is chromosomal segment of length 125Kb containing metBJF and argECBH operons, is unusually unstable. The parent of this KLF5 is an Hfr donor Ra-2, an E.coli K-12 derivative in which F is integrated into the chromosome at a site, probably gamma delta which is proximal to the oriC. Such an integration of F occurs by a cross-over between gamma delta of F and the copy of gamma delta prevailing in the chromosome (sfa site). Duplication of gamma delta (Tn1000), one copy at each junction of the F and the E.coli K12 chromosome. The Hfr Ra-2 thus formed is unstable. F factor becomes RTF first by the self- deletion effect of gamma –de... view more»

DOI: 10.4172/lpma.1000246

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