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Denis Clay Author
Subjects of specialization
Affiliation
Applied Microbiology, Applied Microscopy, Applied Mycology, Applied Physiology, Biomedical Research
University Paris Saclay in France
Biography
Denis Clay was persuing research field in Inserm Research centre . He did Phd from university of paris saclay in france.He has kind of interests in Applied Microbiology, Applied Microscopy and more..
Publications
Short Communication Open Access
Generation of a bi-potent hemangiogenic progenitor from human pluripotent stem cells
Author(s): Alejandra Vargas Valderrama, Denis Clay, Georges Uzan, Hind Guenou and Maria Teresa Mitjavila Garcia
The close temporal-spatial relationship between hematopoietic and endothelial cells during early embryonic development has led to the hypothesis of a common ancestor, the hemangioblast. Here human pluripotent stem cells (hPSCs) endothelial and hematopoietic differentiation via a bipotent progenitor and an in vitro equivalent of the hemangioblast were explored. hPSCs were differentiated into hemangiogenic mesoderm through embryoid body (EB) formation under serum-free culture conditions. After three, five days of differentiation, a population positive for CD309 (VEGFR-2), CD144 (VE-Cadherin), CD143 (ACE) and CD34 was isolated based on the expression of CD144 and cultured either under endothelial or hematopoietic conditions.
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