Eva Cuypers Author

Research Article Open Access

Screening of Urine and Blood Using Limited Sample Preparation and Information Dependent Acquisition with LC-MS/MS as Alternative for Immunoassays in Forensic Toxicology

Author(s):

Ruth Verplaetse, Sylvie Decabooter, Eva Cuypers and Jan Tytgat

Screening of Urine and Blood Using Limited Sample Preparation and Information Dependent Acquisition with LC-MS/MS as Alternative for Immunoassays in Forensic Toxicology

Immunoassays are widely used to perform an initial toxicological screening of biological samples. However, LC-MS/MS is described as a promising technique that can overcome the limitations of immunoassays (such as their lack of selectivity). The objective of this project was to implement a LC-MS/MS method for screening of forensic ante- and post-mortem urine and whole blood samples that can replace the immunoassays. Easy and rapid sample preparation techniqueswere evaluated. Protein precipitation with acetonitrile combined with aqueous dilution (dilution factor 5 for urine and 10 for blood) proved... view more»

DOI: 10.4172/2325-9841.1000112

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