Fredj NB Author

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Development and Validation of a Chromatographic Method for the Quantification of Isoniazid in Human Plasma

Author(s):

Alshaikheid M, Fredj NB, Chaabane A, Chadli Z, Slama A, Boughattas N, Lassoued MA, Aouam K

Objective: Isoniazid (INH) is a major antituberculosis treatment, characterized by a narrow therapeutic index and a large within and between-patient variability. Thus, Therapeutic Drug Monitoring (TDM) of this drug is mandatory in order to optimize the efficacy and minimize occurrence of toxic side effects of this drug. The aim of the present study is to develop and validate a simple chromatographic method for determination of INH in human plasma.

Methods: High performance liquid chromatography was performed using a chromatograph Ultimate 3000®. The extraction of INH from plasma was realized using trichloroacetic acid (TCA). Nicotinamide was used as an internal standard (IS). The mobile phase consiste... view more»

DOI: 10.4172/2325-9604.1000177

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