George Uchenna Eleje2 Author

Research Article Open Access

Allele Frequency Associated With Dhfr And Dhps Genes Obtained From Plasmodium vivax In Anambara State, Nigeria

Author(s):

Isaac Okezie Godwin¹ , Ifeoma Mercy Ekejindu1 , George Uchenna Eleje² , Alfred Friday Ehiaghe¹ , Bright Chukwuebuka Unaeze¹ and George Oche Ambrose^{3 *}

The Plasmodium vivax dihydrofolate reductase (DHFR) and dihydropteroate synthetase (DHPS) are enzymes of central importance in parasite metabolism. The dhfr and dhps gene mutations are known to be associated with sulphadoxine/ pyrimethamine (SP) resistance. The aim of this study is to investigate the single nucleotide polymorphisms (SNPs) associated with these mutations and to determine their nature and distributions. A total number of 390 pregnant women were recruited, 336 of them were taking SP while 54 of the pregnant women were using other anti-malaria drugs for treatment of malaria. The polymerase chain reaction (PCR) technique was used to characterize the specie of the isolated plasmodium while Sanger sequencing method of molecular genotyping was adopted for the subjection of the ... view more»

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