Homa Faghihi Author

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Improvement of Aerodynamic Behavior of Spray-Freeze Dried IgG Powder Applying Amino Acids

Author(s):

Shabnam Aghababaie, Fakhrossadat Emami, Maryam Amini Pouya, Behnaz Daneshmand, Maryam Sadat Mirmoeini, Homa Faghihi and Alireza Vatanara

Introduction: Improved stabilization efficiency of processedpowders has been observed via spray freeze drying (SFD) of antibody dried-formulations. The competence of different amino acids was compared with special focus on aerodynamic properties.

Methods: Trehalose in 1:1 of mass ratio to IgG besides Leucine, Phenyl alanine and Glycine in 1:4 and 1:8 of mass ratio to IgG were prepared through SFD. Size-exclusion chromatography (SEC-HPLC) employed to determine the soluble aggregates. Twin stage impinger (TSI), laser light scattering and scanning electron microscopy (SEM) utilized to characterize particle properties. Powder density and thermal behavior analyzed through graduated glass cylinder and differential scanning calorimetry (DSC).

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