Mahmoud Mohammad Author

Research Article Open Access

HPLC Profiles of Onion Fructooligosaccharides and Inulin and their Prebiotic Effects on Modulating Key Markers of Colon Function, Calcium Metabolism and Bone Mass in Rat Model

Author(s):

Hoda Mabrok, Magda Soliman, Mahmoud Mohammad and Laila Hussein

The objective of this study was to purify and characterize the fructooligosaccharides (FOS) and inulin (INU) contents in local onion varieties. Vigorous extraction of the onion with hot water recovered over 93% of the initial non digestible carbohydrates (NDC). HPLC separation and quantitation showed that total FOS accounted for 20% and INU for 16% of the dry onion NDC extract. The prebiotic effect of onion NDC was tested by incorporating the freeze dried onion NDC at 13% in semisynthetic diet fed to Sprague Dawley rats for 5 weeks. Commercial FOS and INU were run in parallel and served as references. Key markers of colon function included measuring enlargement in colon weight as criteria of bifidogenic effect and colonization, activities of the caecal glyco... view more»

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