Journal of Veterinary Science & Medical DiagnosisISSN: 2325-9590

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Megha Kadam Bedekar Author

meghkadam@gmail.com

Subjects of specialization
Secretory expression system

Affiliation
Genetic engineering of Bacteria Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izat Nagar, Bareilly, Uttar Pradesh-243122, India

Biography

 Megha Kadam Bedekar Belonging to the Department of Genetic engineering of Bacteria Lab, Division of Veterinary Biotechnology, in the field  Indian Veterinary Research Institute, Izat Nagar, Bareilly, Uttar Pradesh-243122, India

Publications

Research Article Subscription

Protection against P. multocida B:2 using Recombinant 87kDa OMP Periplasmic Protein

Author(s):

M. Bagath, Megha Kadam Bedekar, Pallab Chaudhuri, Varalakshmi S and P. P. Goswami

Protection against P. multocida B: 2 using Recombinant 87kDa OMP Periplasmic Protein

To clone and express Pasteurella. multocida (P. multocida) encoding 87 kDa omp gene in prokaryotic (secretory) vector and to study the immunogenicity of expressed protein.The genomic DNA was isolated from P. multocida B:2. Specific primers were designed to suite secretory prokaryotic vector system. PCR amplified gene was cloned in pGEMT-EASY vector (Promega, USA) and subcloned into pBAD/gIIIA prokaryoti... view more»

DOI: 10.4172/2325-9590.1000151

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