Moses Olubusuyi Adewumi Author
Subjects of specialization
Affiliation
Infection, Immunoblotting, Microbiology, Immunology of Infectious Diseases, ELISA
Department of Virology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
Research Article Subscription
Author(s): Johnson Adekunle Adeniji, Adetunji Oladapo Adewale, Temitope Oluwasegun Cephas Faleye and Moses Olubusuyi Adewumi
In 2010, we described sewage contaminated water (SCW) bodies that consistently yielded enteroviruses (EVs) in enterovirus surveillance (ES) sites in Lagos, Nigeria. By 2012, we demonstrated the presence and circulation of Wild Poliovirus 3 (WPV3) in these ES sites. Here we describe ES sites that consistently yield EVs in Ibadan metropolis southwest Nigeria.
Twenty-five ES samples were collected by grab method from nine sites between October, 2012 and March, 2013. Samples were concentrated and four (RD, HEp2C, MCF-7 and L20B) different cell lines used for virus isolation from the concentrates. Isolates were subjected to RNA extraction, cDNA synthesis, PanEnterovirus 5l-UTR and VP1 assays. Unidentifiable isolates were furth... view moreĀ»