Nicolas Nafati Author

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Application of Diagnostic Indicators for Biological Test Validation in Assisted Reproductive Technology

Author(s):

Nicolas Nafati, Ounissa Ait-Ahmed and Samir Hamamah

In the field of medical reproductive research, the selection of embryos with the best potential for implantation is the main challenge for biologists. Several studies suggest that genes involved in the Oocyte-Cumulus Cell crosstalk could represent candidate genebiomarkers for selecting embryos with the highest implantation potential.Therefore, the principle objective of this study is to verify the transcriptomic experimental data from 21 biomarker genes by RT-qPCR (Real-Time quantitative Polymerase Chain Reaction) of 102 embryonic/cumulus cell samples from patients undergoing in vitro fertilization. Since variability (noises) from various sources (biological, technical, etc.) was observed,there is a reasonable doubt about the capability of these transcriptomic data to provide a reliable... view more»

DOI: 10.4172/2329-9533.1000148

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