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Sanad Alonezi Author
Subjects of specialization
Affiliation
Applied Microbiology, Applied Microscopy, Applied Mycology, Applied Physiology, Biomedical Research
Prince Sultan Military Medical City, Saudi Arabia
Biography
Sanad Alonezi received his Bachelor’s Degree in Pharmaceutical Sciences from KSU in 2003 and MSc from KSU, Saudi Arabia. He holds PhD in Pharmaceutical analysis from university of Strathclyde in 2017 (UK). He is a consultant pharmacist at PSMMC. He has a strong interest in areas of research involve high resolution mass spectrometry and chromatography applied to metabolomics.
Publications
Short Communication Open Access
The effects of cisplatin on the metabolome of ovarian cancer cells by using LC-MS
Author(s): Sanad Alonezi
In this work, liquid chromatography-mass spectrometry (LC-MS) with zwitterionic HILIC material (ZIC-pHILIC) was used to analyze the intracellular metabolomic spectrum of the human ovarian cancer cell line A2780 and the cisplatin resistance cell line A2780CR, in which cisplatin was used to treat the cells at IC50 and IC20 concentrations. The cisplatin-sensitive cells were as expected more sensitive to cisplatin than the resistant cells with IC50 values of 4.9 and 10.8 μg/mL, respectively. Data extraction was carried out with MZmine 2.14 with metabolite searching against an in-house database. The data were analyzed using the univariate and multivariate principal component analysis (PCA) and Orthogonal projections to latent structures discriminant analysis (OPLS-DA) methods. There was c... view more»
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