Shalaby UA Author

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The Role of Extracellular Matrices in Retinal Pigment Epithelial Cell Migration

Author(s):

Shalaby UA, Saeed AM and Elmohamady MN

Background: To identify which extracellular matrix components modify retinal pigment epithelial cell migration and their interplay with matrix metalloproteinases in directing the behaviour of the cells in agarose droplets, an in-vitro model for cellular migration.

Methods: Migration of the cells from agarose droplets on coated tissue culture plastic (fibronectin, laminin and collagen types: I and IV) in the presence as well as absence of serum was evaluated by using phase contrast microscopy and computer analysis of captured images. Expression of metalloproteinases -1, 2 and 9 by retinal pigment epithelial cells was determined by immunocytochemistry and was confirmed by western blotting. In addition, the effect of metalloproteinases inhibitor,... view more»

DOI: 10.4172/2324-8599.1000206

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