Sylvie Decabooter Author
Subjects of specialization
Affiliation
Screening
University of Leuven, KU Leuven, Toxicology and Pharmacology, Campus Gasthuisberg O&N 2, Herestraat 49, 3000 Leuven, Belgium
I am Sylvie Decabooter, belonging to University of Leuven, KU Leuven, Toxicology and Pharmacology, Campus Gasthuisberg O&N 2, Herestraat 49, 3000 Leuven, Belgium, interested in the field of Screening.
Research Article Open Access
Author(s): Ruth Verplaetse, Sylvie Decabooter, Eva Cuypers and Jan Tytgat
Screening of Urine and Blood Using Limited Sample Preparation and Information Dependent Acquisition with LC-MS/MS as Alternative for Immunoassays in Forensic Toxicology
Immunoassays are widely used to perform an initial toxicological screening of biological samples. However, LC-MS/MS is described as a promising technique that can overcome the limitations of immunoassays (such as their lack of selectivity). The objective of this project was to implement a LC-MS/MS method for screening of forensic ante- and post-mortem urine and whole blood samples that can replace the immunoassays. Easy and rapid sample preparation techniqueswere evaluated. Protein precipitation with acetonitrile combined with aqueous dilution (dilution factor 5 for urine and 10 for blood) proved... view moreĀ»