Yogesh JC Author

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Simultaneous Quantitation of Three Genotoxic Impurities in Rizatriptan Benzoate Using Liquid Chromatography-Mass Spectrometry

Author(s):

Niranjan MD, Manish GG, Yogesh JC, Nelson R D’souza, Navin VD and Jaydeep NG

A selective, sensitive and high throughput Liquid Chromatography Mass Spectrometry method has been developed for simultaneous quantification of three genotoxic impurities in Rizatriptan Benzoate. Three genotoxic impurities of Rizatriptan Benzoate namely 1-(4-Nitrobenzyl)-1,2,4-triazol, 4-(1H-1,2,4-Triazol1-yl methyl) benzene amine and 4-Amino-1-(4-nitrobenzyl)-1,2,4-triazolium bromide were separated on Zorbax SB-CN (150 mm × 4.6 mm, 3.5 µm) column using 10 mM ammonium acetate as buffer and acetonitrile as organic modifier in gradient composition at the flow rate of 1.0 ml/ min. All the impurities exhibited linearity with correlation of coefficient value greater than 0.990. The method was validated over the concentration range of 5 µg/g-70 &... view more»

DOI: 10.4172/2325-9604.1000163

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