Yoshiyuki Koyama Author

Research Article Open Access

Effect of pDNA Complex Particle Size on Gene Expression: Difference between In Vitro and In Vivo Experiments

Author(s):

Tomoko Ito, Masazumi Eriguchi and Yoshiyuki Koyama

Effect of pDNA Complex Particle Size on Gene Expression: Difference between In Vitro and In Vivo Experiments

We have developed a novel gene-transfection system comprising very small (70-150 nm) plasmid/polyethyleneimine/hyaluronan (HA) ternary complexes having negative surface charge, and reduced nonspecific interactions with bio-components. They showed high in vivo gene expression efficiency. However, in in vitro transfection experiments, such small ternary complexes often demonstrated rather much lower gene expression than the conventional DNA/ polycation complex. In this study, we investigated the influence of the complex particle size on gene expr... view more»

DOI: 10.4172/2324-8777.1000202

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