Journal of Otology & RhinologyISSN: 2324-8785

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Research Article, J Otol Rhinol S Vol: 4 Issue: 1

Changes in Contents of Arachidonic Acid Metabolites in Nasal Lavage and Mucosal Tissues in response to Allergen Challenge in Patients with Allergic Rhinitis

Masato Miwa1*, Daisuke Sasaki1, Mikio Hirotsu1, Noritsugu Ono1, Akihito Shiozawa1, Mayumi Miwa2 and Katsuhisa Ikeda1
1Department of Otorhinolaryngology, Juntendo University Faculty of Medicine, Tokyo, Japan
2Harimazaka Clinic, 4-20-2 Koishikawa, Tokyo, Japan
Corresponding author : Masato Miwa, MD
2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan
Tel: +81-3-5802-1094; Fax: +81-3-5689-0547
E-mail: [email protected]
Received: November 17, 2014 Accepted: January 17, 2015 Published: March 09, 2015
Citation: Miwa M, Sasaki D, Hirotsu M, Ono N, Shiozawa A, et al. (2015) Changes in Contents of Arachidonic Acid Metabolites in Nasal Lavage and Mucosal Tissues in response to Allergen Challenge in Patients with Allergic Rhinitis. J Otol Rhinol S1:1. doi:10.4172/2324-8785.S1-005


Background: Allergic rhinitis is recognized as being not purely a histamine-related disease. Three arachidonate metabolites, i.e. leukotriene (LT), thromboxane (TX) A2 and prostaglandin (PG) D2, have gathered more interest because of increasing evidence supporting a causal relationship for these mediators. We attempted to evaluate changes in the contents of these three mediators not only in nasal lavage but also in mucosal tissue.

Methods: The contents of three arachidonic acid metabolites; PGD2, TXB2, LT C4, D4 and E4 (LTs), in nasal lavage and nasal mucosal tissues in subjects with allergic rhinitis after antigen challenge were measured.

Results: The highest level of these three mediators in nasal lavage was achieved at 15 min after challenge. In most subjects that showed the late NAR response, an apparent second mediator rise was observed. While the secondary rise of PGD2 and TXB2 was seen from 4 to 8 hr. after the challenge at which the NAR response was seen, that of LTs was seen 2 to 4 hr. after the challenge and preceded the late NAR response by about 2 hrs.

In the non-allergic subjects, the contents of PGD2, TXB2, and LTs were not significantly altered by the exposure to HD allergen. In the challenged side of the allergic subjects, the contents of all the three mediators were significantly high.

Conclusion: The relative content of the three arachidonic acid metabolites in the mucosa after provocation was found to be different from that observed in the lavage fluid; the amount found in the lavage does not reflect the relative amount formed in the mucosa.

Keywords: Human nasal mucosa; Prostaglandin D2; Thromboxane A2; Leukotriene C4, D4 and E4; Nasal provocation; Allergic rhinitis; Allergen challenge

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