Journal of Proteomics & EnzymologyISSN: 2470-1289

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Research Article, J Biocatal Biotransformation Vol: 2 Issue: 2

Enhancing Thermal Stability of Immobilized Raw Starch Digesting Amylase (RSDA)Using Different Additives

Onyetugo C Amadi1,2, Bartho N Okolo2, Cesar Mateo1, Jose M Guisan1 and Benevides C Pessela3*
1Departamento de Biocatálisis Enzimática, Instituto de Catálisis y Petroleoquímica, ICP-CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, España
2Department of Microbiology, Faculty of Biological Sciences, University of Nigeria Nsukka, Nigeria
3Departamento de Microbiología y Biotecnología, Instituto de Investigaciones en Ciencias de los alimentos, CIAL-CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, España
Corresponding author : Dr. Benevides C Pessela
Departamento de Microbiología y Biotecnología, Instituto de Investigaciones en Ciencias de los alimentos, CIAL-CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, España
Tel: +34910017962; Fax: +34910017905
E-mail: [email protected]
Received: October 30, 2013 Accepted: December 11, 2013 Published: December 18, 2013
Citation: Amadi OC, Okolo BN, Mateo C, Guisan JM, Pessela BC (2013) Enhancing Thermal Stability of Immobilized Raw Starch Digesting Amylase (RSDA) Using Different Additives. J Biocatal Biotransformation 2:2. doi:10.4172/2324-9099.1000109

Abstract

Enhancing Thermal Stability of Immobilized Raw Starch Digesting Amylase (RSDA) Using Different Additives

Industrial and large scale applications of biocatalysts are often limited by lack of thermostability. Raw starch digesting amylase(RSDA) of Aspergillus carbonarius was immobilized on cyanogen bromide to facilitate mild covalent attachment. To improve thermal stability of immobilized and soluble RSDA different concentrations of sugar (trehalose) and polyols (polyethylene glycol, glycerol and mannitol) under varying conditions; temperature, pH and buffer ionic strength was studied.

Keywords: Aspergillus carbonarius; Thermostability; Immobilized enzyme; Polyols; Sugar

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