Endocrinology & Diabetes ResearchISSN: 2470-7570

Reach Us +12097300872
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article, J Physiobiochem Metab Vol: 3 Issue: 1

Exhaled Breath Aspiration Ion Mobility Spectrometry Profiles Reflect Metabolic Changes Induced By Diet

Kaisa Raninen1*, Mikko Kolehmainen2, Tomi-Pekka Tuomainen1, Hannu Mykkänen1, Kaisa Poutanen1 and Olavi Raatikainen1
1Institute of Public Health and Clinical Nutrition, University of Eastern Finland
2Department of Environmental Sciences, University of Eastern Finland
Corresponding author : Kaisa Raninen
Institute of Public Health and Clinical Nutrition, University of Eastern Finland, PO Box: 1627, FI-70211 Kuopio, Finland
Tel: ++358 403552906
E-mail: [email protected]
Received: October 09, 2014 Accepted: November 19, 2014 Published: November 21, 2014
Citation: Raninen K, Kolehmainen M, Mykkänen H, Poutanen K, Raatikainen O (2015) Exhaled Breath Aspiration Ion Mobility Spectrometry Profiles Reflect Metabolic Changes Induced By Diet. J Physiobiochem Metab 3:2. doi:10.4172/2324-8793.1000116

Abstract

Breath analysis has been developed for quantification of individual compounds e.g. in disease diagnostics, but new technologies allow also on-line measurement of metabolite profiles. We evaluated the potential of monitoring diet induced changes in metabolism based on breath profiles analyzed by aspiration ion mobility spectrometry (AIMS). Exhaled breath alveolar samples were taken at fasting state after low versus high fibre diets (17 and 44 g/day for one week in randomized order, n=7) and during an aerobic exercise test (30 minutes cycling with cycle ergometer, n=9) and analyzed with ChemPro®100, a hand-held gas detector based on AIMS. Principal component analysis was first carried out as pre-processing and next Hotelling’s T-test and linear discriminate regression models (LDRM) were applied to discriminate the exhaled breath profiles in different metabolic states. The exhaled breath profiles were different between the diets (Hotelling´s test, p= 0.015, LDRM: R2=0.71, sensitivity 0.71, specificity 0.71 and accuracy 0.71) and between the resting and the exercise states (p=0.012, R2= 0.79, sensitivity 1.00, specificity 0.89 and accuracy 0.94).This indicates the potential of utilizing the exhaled breath profiles for detecting dietary changes and monitoring exhaled breath further in studying the mechanisms of dietary effects.

Keywords: Aspiration Ion Mobility Spectrometry; Breath Profile; Exhaled breath; Diet; Dietary fibre; Exercise

Track Your Manuscript

Share This Page

Media Partners