Endocrinology & Diabetes ResearchISSN: 2470-7570

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Research Article, J Physiobiochem Metab Vol: 1 Issue: 2

Gamma-Tocopherol Induces Apoptotic Cell Death in Human Adenoid Cystic Carcinoma Cells Derived from a Salivary Gland Tumor

Abstract

Gamma-Tocopherol Induces Apoptotic Cell Death in Human Adenoid Cystic Carcinoma Cells Derived from a Salivary Gland Tumor

This study investigated both the possible antiproliferative and proapoptotic effects of, either alpha-tocopherol or gammatocopherolin vitro, upon a human salivary gland cancer cell line(ACC3), derived from an adenoid cystic carcinoma and M1 cells, a non-cancerous human salivary gland cell line. Prior to the use of these cell lines for treatment, they were examined histologically, to confirm their origin as human salivary gland cells. When ready for experimental use, cells were grown in a final concentration of 0, 10, 25 or 50 micromoles/liter of, either alpha-tocopherol or gamma-tocopherol, and evaluated for growth at 72 hours. Cells were analyzed for their proliferative activity by an assay, which uses 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), a yellow tetrazole which is reduced to purple formazan in living cells.

Keywords: Gamma-tocopherol; Vitamin E; Apoptosis; Alphatocopherol

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