Journal of Proteomics & EnzymologyISSN: 2470-1289

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Research Article, J Biocatal Biotransformation Vol: 2 Issue: 2

Isolation, Purification and Characterization of Fungal Extracellular L-Asparaginase from Mucor hiemalis

Monica Thakur, Lynette Lincoln, Francois N Niyonzima and Sunil S More*
Department of Biochemistry, Center for Post Graduate Studies, Jain University, Bangalore, India
Corresponding author : Sunil S More
Department of Biochemistry, CPGS, Jain University, 18/3, 9th Main Jayanagar 3rd Block, Bangalore-560011, India
Tel: +91 9481787729; Fax: +91 080 43226501
E-mail: [email protected]
Received: September 20, 2013 Accepted: December 12, 2013 Published: December 23, 2013
Citation: Monica T, Lincoln L, Niyonzima FN, Sunil SM (2013) Isolation, Purification and Characterization of Fungal Extracellular L-Asparaginase from Mucor Hiemalis. J Biocatal Biotransformation 2:2. doi:10.4172/2324-9099.1000108

Abstract

Isolation, Purification and Characterization of Fungal Extracellular L-Asparaginase from Mucor hiemalis

L-asparaginase is an enzyme that deaminates the free L-asparagine to yield aspartic acid and is used as an antileukemic agent. L-asparaginase producing fungus was screened from a local soil sample and identified as Mucor hiemalis based on morphological and microscopic characteristics.

Keywords: Mucor hiemalis; L- asparaginase;Non metalloprotein; Lectinagarose column; Antioxidant

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