Journal of Proteomics & EnzymologyISSN: 2470-1289

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Research Article, J Biocatal Biotransformation Vol: 2 Issue: 1

Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis

Jun Cai1*, Jin Li2, Changgao Wang1, Jianguo Lin1, Ying Hu1, Jianhong Yang3, Yumin Du4 and Hua Zheng5
1Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China
2College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
3Department of Environmental Engineering, School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China
4Department of Environmental Science, College of Resource and Environmental Science, Wuhan University, Wuhan 430079, Wuhan, China
5Department of Pharmaceutical Engineering, School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China
Corresponding author : Jun Cai
Key Laboratory of Fermentation Engineering (Minister of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China
Tel: +86-27-88032319; Fax: +86-27-88032320
E-mail: [email protected]
Received: February 04, 2013 Accepted: February 22, 2013 Published: February 28, 2013
Citation: Cai J, Li J, Wang C, Lin J, Hu Y, et al. (2013) Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis. J Biocatal Biotransformation 2:1. doi:10.4172/2324-9099.1000103

Abstract

Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis

The culture medium, fermentation conditions and effects of different inorganic salts on producing extracellular chitin deacetylase by Scopulariopsis brevicaulis were optimized.The highest enzymatic activity of deacetylation of chitin was 36 units/mL for optimum culture medium which included 2% (w/v) 3,6-O-carboxymethylchitin, 1% sucrose, 0.2% NaNO3, 0.1% K2HPO4, 0.05% KCl, 0.4% peptone, 0.05% MgSO4, 0.05% MnSO4, 0.03% CoCl2 and optimum cultural conditions included temperature, pH, agitation speed, fermenting time and volume and were 29°C, 7.0, 200 rmp min-1, 96 hours and 80 mL in 300 mL Erlenmeyer flask, respectively

Keywords: Chitin; Extracellular chitin deacetylase; Parametric optimization; Scopulariopsis brevicaulis

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