Journal of Clinical & Experimental OncologyISSN: 2324-9110

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Research Article, J Clin Exp Oncol Vol: 5 Issue: 1

A Methylation Profile of Circulating Cell Free DNA for the Early Detection of Gastric Cancer and the Effects after Surgical Resection

Dong Gue Shin1, Kyong chol Kim2*, Sang-Woon Choi2,3, Hee Jae Joo4, Seung Hyuk Baik5 and Min Koo Park6
1Department of General Surgery, Red Cross Hospital, Saemunan Ro 9, Jongro Gu, Seoul, Korea
2Chaum Life Center, CHA University, Dosan DaeRo 442, Kangnam Gu, Seoul, Korea
3Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston
4TCM Epigenetics and Genomics Laboratory, Yoksamro 230, Kangnamgu, Seoul, Korea
5Department of Surgery, Yonsei University College of Medicine, Yonseiro 50, Seodaemungu, Seoul, Korea
6Nutrigenetics Institute, Erom, co. Ltd, Toegenonggongro 111, Chun Cheon, South Korea
Corresponding author : Kyong chol Kim
Chaum Life Center, CHA University, Dosan DaeRo 442, Kangnam Gu, Seoul, Korea 135-948
Tel: +82-2-3015-5330; Fax: +82-2-3015-5336
E-mail: [email protected]
Received: January 27, 2015 Accepted: February 05, 2016 Published: February 09, 2016
Citation: Shin DG, Kim KC, Choi S, Joo HJ, Baik SH, et al. (2016) A Methylation Profile of Circulating Cell Free DNA for the Early Detection of Gastric Cancer and the Effects after Surgical Resection. J Clin Exp Oncol 5:1. doi:10.4172/2324-9110.1000151

Abstract

Circulating cell free DNA (cfDNA) has been suggested as a novel biomarker for the early detection of cancer. Therefore, we investigated whether the methylation pattern of promoters of critical genes in cfDNA can be a useful marker for the early detection of gastric cancer and the therapeutic effects of treatment. In this casecontrol study, the methylation status of 32 cancer associated genes from 41 gastric cancer patients and 104 healthy control subjects were measured by methylation-specific PCR. We found that 15 out of 32 cancer-associated genes were hypermethylated in cancer patients compared to those of the controls. Further, we found that a gene panel of PYCARD, APAF1, MINT1, and BRCA1 genes showed 97.6% sensitivity and 66.3% specificity for the presence of gastric cancer. Lastly, we found that 22 initially methylated genes became unmethylated after surgical resection of the tumor (p< 0.05). The aberrant methylation pattern of tumor suppressor genes can be reliable biomarkers for the early detection of gastric cancer and of the efficacy of surgical resection.

Keywords: DNA methylation; Circulating cell free DNA (cfDNA); Biomarker; Gastric cancer

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