Journal of Pharmaceutics & Drug Delivery Research ISSN: 2325-9604

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Research Article, J Pharm Drug Deliv Res Vol: 5 Issue: 3

Evaluation of Insulin Release from Alginate Nanoparticles by Two Different Methods

Mohammadi Rovshandeh J1, Aghajamali M2, Haghbin Nazarpak M3* and Toliyat T4
1Department of Chemical Engineering, College of Engineering, University of Tehran, Rezvanshahr, Iran
2Department of Chemistry, College of Science, University of Tehran, Tehran, Iran
3New Technologies Research Center (NTRC), Amirkabir University of Technology, Tehran, Iran
4Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Corresponding author : Haghbin Nazarpak M
New Technologies Research Center (NTRC), Amirkabir University of Technology, Tehran, 15875-4413, Iran
Tel: +98 (21) 64540
E-mail: [email protected]
Received: July 12, 2015 Accepted: August 06, 2016 Published: August 09, 2016
Citation: Mohammadi Rovshandeh J, Aghajamali M, Haghbin Nazarpak M, Toliyat T (2016) Evaluation of Insulin Release from Alginate Nanoparticles by Two Different Methods. J Pharm Drug Deliv Res 5:3. doi:10.4172/2325-9604.1000150

Abstract

Evaluation of Insulin Release from Alginate Nanoparticles by Two Different Methods

Abstract

In this work, insulin was encapsulated within alginate nanoparticles by emulsification and spraying methods to preserve insulin at simulated gastric pH. Mean diameter and size distribution of nanoparticles were characterized by particle size analyzer. Morphology of particles was investigated by scanning electron microscopy. Insulin encapsulation efficiency and in vitro release under simulated gastrointestinal conditions were determined by insulin ELISA test kit. Encapsulation efficiency was calculated 20% and 58% for nanoparticles prepared by spraying and emulsification method, respectively. The mean diameter of alginate nanoparticles ranged from 90.12-99.15 nm for particles prepared by spraying method and 100.92 - 111.11 nm for particles prepared by emulsification method. Insulin release was measured at different pH; at pH 1.2 no insulin release was observed, however by increasing the pH value up to 6.8, maximum insulin release was observed after 30 min from both nanoparticles. These results show that the nanoparticles could be used for oral delivery of insulin, as they completely preserve insulin at simulated gastric pH and could release insulin at simulated intestinal pH.

Keywords: Alginate; Insulin; Emulsification method; Spraying method;Nanoparticles; Oral delivery

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