Journal of Pharmaceutics & Drug Delivery Research ISSN: 2325-9604

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Research Article, J Pharm Drug Deliv Res Vol: 5 Issue: 3

Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase and Α-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia

Khaled Athmouni1,2*, Taheni Belghith1,2, Abdelfattah El Feki2 and Habib Ayadi2
1Faculty of Sciences of Sfax, University of Sfax, Department of life Sciences, de research Unit UR 11 ES 72/Biodiversity and Aquatic Ecosystems, Road Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia
2Faculty of Sciences of Sfax, Laboratory of Animal Ecophysiology, University of Sfax, P.O. Box 95, Sfax 3000, Tunisia
Corresponding author : Khaled Athmouni
University of Sfax, Faculty of Sciences of Sfax, Department of life Sciences, de research Unit UR 11 ES 72/Biodiversity and Aquatic Ecosystems, Road Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia
Tel: +216 93242084
Fax: +216 74274437
E-mail: [email protected]
Received: March 24, 2016 Accepted: September 12, 2016 Published: September 15, 2016
Citation: Athmouni K, Belghith T, Feki AE, Ayadi H (2016) Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase andΑ-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia. J Pharm Drug Deliv Res 5:3. doi:10.4172/2325-9604.1000152

Abstract

Solvent Effect on Phenolic Compounds Extraction, Antioxidant Capacities and Α-Amylase and Α-Glucosidase Inhibition of Clematis flammula and Periploca angustifolia

Abstract

Phytochemicals are extensively found at different levels in many medicinal plants. This work had three objectives: the first, to evaluate the total phenolic, flavonoids content, anthocyanins concentration and condensed tannins of two Tunisian medicinal plants, second, to assess their potential antioxidant activities using systems such as a, adiphenyl-b-picrylhydrazyl (DPPH• ), ABTS•+, O2 ?• , H2 O2 scavenging activity, Hydroxyl OH• and linoleic acid inhibition and third, to screen α-amylase and α-glucosidase inhibitors from common natural products found in traditional Tunisian medicines. The present study was performed that C. flammula (Ranunculaceae family)has the highest levels of total phenols followed by P. angustifolia (Asclepiadaceae family). On the other hand, the results show that the antiradical activity (DPPH) varies from 52 to 77% for P. angustifolia and 56 to 84% for C. flammula. The antioxidant activity measurement, expressed as Trolox equivalent antioxidant capacity (TEAC), ranged from 0.33 to 1.51 mM TE/ g DW. In general, all extracts exhibited good antioxidant activity. In the present investigation, the extracts most effective in inhibiting α-amylase (C. flammula and P. angustifolia) contain appreciable amounts of condensed tannins. In addition, the extent of inhibition of α-glucosidase was related to their anthocyanins content.

Keywords: C. flammula; P. angustifolia; Antioxidant activity; α-amylase; α-glucosidase

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