Journal of Virology & Antiviral ResearchISSN: 2324-8955

Research Article, J Virol Antivir Res Vol: 4 Issue: 4

Two Isolates of Potato Virus Y (PVY) and the Response of Different Potato Cultivars Against the Viral Infection

Dalia Gamil Aseel1, Azaa Makhlouf2, Sanaa A Riad2, Elmorsi AA3, GI Fegla4 and Hafez EE1*
1Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Borg EL-Arab city, Alexandria, Egypt
2Genetics Department, Faculty of Agriculture, Alexandria University, Alexandria,Egypt
3Botany Department, Faculty of Science, Mansoura University, Mansoura,Egypt
4Plant Pathology Department, Faculty of Agriculture, Alexandria University,Alexandria, Egypt
Corresponding author : Elsayed Elsayed Hafez City of Scientific Research and Technology Applications, Arid Lands Cultivation Research Institute (ALCRI), High Institute of Public Health - Alexandria university, 165 New Borg El Arab City, 21934, Alexandria, Egypt
Tel: +203 4595100
E-mail: [email protected]
Received: October 27, 2015 Accepted: December 22, 2015 Published: December 29, 2015
Citation: Aseel DG, Makhlouf A, Riad SA, Elmorsi AA, Fegla G, et al. (2015) Two Isolates of Potato Virus Y (Pvy) and the Response of Different Potato Cultivars Against the Viral Infection. J Virol Antivir Res 4:4. doi:10.4172/2324-8955.1000145

Abstract

Potato virus Y (PVY), of the genus Potyvirus, family Potyviridae, is highly pathogenic virus, causing enormous economic losses in solanaceous crops. Based on the ELISA test for the screened areas, the virus incidence ranged from 15- 30%. Mild mosaic and/ or mottling associated with leaf deformation were observed after 21 days on N. tabacum cv (white barley and galtinosa), mechanically inoculated with PVY- Kafer El-Zayat isolate. However, only mild mosaic was observed on same plants following PVY-Komhamada challenge. Molecular analysis measures including, coat protein amplicons, RFLP, sequence and phylogenetic analysis of the viral CP genes (~900 bp), confirmed that the two PVY isolates are existed in Egypt (Borg El Arab and Kafer El Zayat isolates). These two isolates were in close relation to France (KC005883) and Germany (AJ890342) isolates with identity 95% and 90%, respectively. Our results showed that the potato cultivar Spunta was more resistant to the infection by the PVY and the viral coat protein gene exhibited a low level of expression compared to the other two cultivars, Cara and Diamont. Collectively, our study here demonstrates that the two PVY isolates were commonly distributed in the Egyptian potato fields and their origin are European and Spunta potato cultivar is the recommended cultivar for cultivation in all the examined areas

Keywords: PVY; I-ELISA; RT-PCR; Phylogenetic tree

Track Your Manuscript

Share This Page

Media Partners