Antiviral activity of Aristolochia bracteolata against influenza A virus
Mona Timan Idriss, Malik SulimanMohamed, Sarawut Khongwichit, Natthida Tongluan, Duncan R Smith, N H Abdurahman and Alamin Ibrahim
Elnima
Sudan International University, Sudan
Khartoum University, Sudan
Mahidol University, Thailand
Universiti Malaysia Pahang, Malaysia
: J Immunol Tech Infect Dis
Abstract
We investigated the anti-influenza virus activity of Aristolochia bracteolate and possible mechanism(s) of action in vitro. We found that Aristolochia bracteolate has anti-influenza-virus activity, and both pre-incubation of virus prior to infection and post-exposure of infected cells with Aristolochia bracteolate extract significantly inhibited virus yields. Influenza-virusinduced hemagglutination of chicken red blood cells was inhibited by Aristolochia bracteolate extract treatment, suggesting that Aristolochia bracteolate can inhibit influenza A virus infection by interacting with the viral hemagglutinin. Furthermore, Aristolochia bracteolate extract significantly affect nuclear transport of viral nucleoprotein (NP). To best of our knowledge, this study revealed for the first time that Aristolochia bracteolate extract can inhibit both viral attachment and replication and offers new insights into its underlying mechanisms of antiviral action. The whole plant of Aristolochia bracteolate was collected from Sudan and extracted with 70% methanol. The crude extract was screened for its cytotoxicity against MDCK cell line by PrestoBlue assay and WST-1 assay. Antiviral properties of the plant extract were determined by cytopathic effect inhibition assay and virus yield reduction assay (plaque assay). Time of addition assay, and nuclear export mechanism were also performed.
Biography
Mona has completed her Master’s degree in Molecular Microbiology of Infectious Disease from Nagasaki University, Japan (2012). Currently, she is working as a Lecture in Faculty of Pharmacy Department of Pharmaceutical Microbiology at Sudan International University.
