Establishing the model of proteomic analysis on the M. pneumoniae
Meng Yan-li
Heilongjiang Academy of Chinese Medicine Sciences, China
: J Immunol Tech Infect Dis
Abstract
Qinbaiqingfei pellets are the first effective Traditional Chinese Medicine approved by the chinese government for the clinical treatment of both drug-sensitive and -resistant strains of Mycoplasma Pneumoniae (M. pneumoniae). We make use of two-dimensional gel electrophoresis (2-DE) method to establish the model of Proteomic map on the M. pneumoniae and explored the mechanisms of the Qinbaiqingfei pellets inhibiting Mycoplasma pneumoniae. Total proteins of 800 μg were extracted from M. pneumoniae with lysis buffer and was dissected with 2-DE (pI 3–11) and visualized by Coomassie blue staining. Protein 2-DE maps of the M. pneumoniae showed a good result. Using 2-DE method to establish the model of Proteomic analysis on the M. pneumoniae laid the groundwork for future studies exploring the mechanisms of this drug.
Biography
Meng Yan-li obtained her Bachelor degree from Harbin medical University and Master degree from Heilongjiang University of the Traditional Chinese Medicine. She has worked in Heilongjiang Academy of Chinese Medicine Sciences as a research assistant since 2008.
E mail: mengyanli2000@163.com
