In vivo studies to assess the protective influence of Lactobacillus plantarum MTCC1325 on Alzheimers disease

<p>Mallikarjuna Nimgampalle and Yellamma Kuna</p>

In vivo studies to assess the protective influence of Lactobacillus plantarum MTCC1325 on Alzheimers disease

Mallikarjuna Nimgampalle and Yellamma Kuna

Sri Venkateswara University, India

: Int J Ment Health Psychiatry

Abstract

The present investigation was aimed to assess the protective effect of Lactobacillus plantarum MTCC1325 against D-Galactose induced Alzheimer’s disease (AD) in male albino rats. Recently, we have demonstrated that L. plantarum modulates the functions of total ATPases and ameliorates the pathological features of AD. In this study, we have evaluated the potential antioxidant nature of L. plantarum through in vitro assays (DPPH and H2O2), then estimated the antioxidant enzymes (SOD, CAT and GR) and lipid peroxidation levels (MDA) in vivo in selected brain regions such as hippocampus and cerebral cortex of male albino rats. Further, the alterations in gene expressions (BDNF and AChE) in the hippocampus of all treated and untreated rats were assessed by quantitative PCR. From the obtained results it was concluded that chronic injection of D-Galactose caused significant impairment of oxidative stress, lipid peroxidation and nerve degeneration in the brain. But the treatment of AD induced rats with L. plantarum for sixty days significantly nullified all above mentioned impairments as compared to AD-Model group. These research findings highlight the protective effects of L. plantarum MTCC1325 against D-Galactose induced oxidative stress, nerve degeneration and variations of BDNF and AChE levels in the AD rat brain.