Journal of Food and Nutritional DisordersISSN: 2324-9323

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

The anti-inflammatory activity of sinapinic acid containing phenolic extracts from Irish rapeseed meal


Leah Quinn

Teagasc Food Research Centre, Ireland

: J Food Nutr Disor

Abstract


Rapeseed meal is a low-economic value by-product of rapeseed oil production, which is commonly used as animal feed. However, rapeseed (Brassica napus L.) contains more phenolic compounds than any other oilseed plant. Sinapinic (SA) has been identified as a major insoluble phenolic in rapeseed hulls and constitutes 70-90% of soluble esterified phenolic acids in rapeseed meal. Phenolic acids, including SA, have known bioactive properties, including ant-inflammatory activity. As part of this project, two phenolic extracts containing SA were generated from Irish rapeseed meal supplied by Donegal Rapeseed Oil Company, Donegal. The anti-inflammatory activity of the extracts were determined. Quantitative Polymerase Chain Reaction (QPCR) and Enzyme-Linked Immunosorbent Assays (ELISA) were performed using THP-1 cells and human primary monocytes. The anti-inflammatory activities of SA containing extracts I and II were determined using two key inflammatory mediators: TNF-alpha and CXCL8. Extract I significantly increased CXCL8 expression but did not affect TNF-alpha expression. Extract II significantly reduced TNF-alpha expression when assayed at concentrations of 1 and 0.5 mg/ml. Extract II at 1 mg/ml also significantly reduced CXCL8 expression, while significantly increasing CXCL8 gene expression. The anti-inflammatory activity of extract II was also assessed using human monocytes over a period of 3 hours, with 1 mg/ml found to significantly reduce CXCL8 and also reduce TNF-alpha expression. Extracts containing SA from Irish rapeseed meal could be potentially valuable as an ant-inflammatory agent.

Biography


E-mail: quinnl7@tcd.ie

Track Your Manuscript

Awards Nomination

GET THE APP