Journal of Proteomics & EnzymologyISSN: 2470-1289

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Research Article, J Biocatal Biotransformation Vol: 2 Issue: 1

Purification, Immobilization, Stabilization and Characterization of Commercial Extract with β-galactosidase Activity

Chiara Calandri1, Daniela P. Marques2, Cesar Mateo4, Alfonso V. Carrascosa3, Jose M. Guisán4, Gloria F. Lorente3* and Benevides C. Pessela3
1Scuela Normale Superiore di Pisa. Piazza dei Cavalieri 7, 56126, Pisa, Italy
2Department of Foods and Nutrition, Faculty of Pharmacy, Universidade Estadual Paulista Julio de Mesquita Filho – UNESP, Highway Araraquara Jau- Km 1, 14801 902, Araraquara, São Paulo, Brazil
3Department of Biotechnology y Food Microbiology, Instituto de Investigaciones en Ciencias de los Alimentos, CIAL, C / Nicolás Cabrera 9, 28049, Cantoblanco, Campus UAM, Madrid, Spain
4Department Biocatálisis, Institute of catalysis-CSIC, Campus UAM. Cantoblanco, 28049 Madrid, Spain
Corresponding author : Dr. Gloria F. Lorente
Department of Biotechnology y Food Microbiology, Instituto de Investigaciones en Ciencias de los Alimentos, Madrid, Spain
Tel: +341 562 2900; Fax: + 341 564 4853
E-mail: [email protected]
Received: January 10, 2013 Accepted: February 14, 2013 Published: February 21, 2013
Citation: Calandri C, Marques DP, Mateo C, Carrascosa AV, Guisán JM, et al. (2013) Purification, Immobilization, Stabilization and Characterization of Commercial Extract with ß-galactosidase Activity. J Biocatal Biotransformation 2:1. doi:10.4172/2324-9099.1000104

Abstract

Tyrosinase and Tyrosinase Inhibitors

From a commercial enzyme extract juice with purpose to stabilize it in the industry, has been able to extract an extract rich in beta-galactosidase activity. It has been designed to extract and purification protocol based on polyethyleneimine SUPPORTS agarose activated with next purification yields 80%.

Keywords: Tyrosinase; Tyrosinase Inhibitors

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