Spherical Gold Nanoparticles Impede the Function of Bovine Serum Albumin In vitro: A New
Consideration for Studies in Nanotoxicology
Suspensions of bovine serum albumin (BSA) and spherical gold nanoparticles were analyzed to determine if gold nanoparticles (nAu) affect the ligand binding properties of BSA. A range of diameters of nAu with a carboxylic acid capping agent (nAu-cap) were tested, along with nanoparticles conjugated to amine (nAu-NH3+) and carboxyl (nAu-COO-) functional groups via a covalent polymer bridge. All nAu tested were found to affect BSA conformation as determined by intrinsic tryptophan fluorescence. Smaller diameters of nAu-cap (30-50 nm), along with nAu-NH3+ and nAu-COO-, impeded the binding of 8-anilino-1-napthalenesulfonic acid (ANS) to BSA. Similarly, smaller diameters of nAu-cap tended to impede oleic acid binding to BSA, with a linear negative correlation observed between nAu-cap diameter and the dissociation constant (KD) of oleic acid over the range of 40-80 nm. 80 nm nAu-cap impeded butanoic acid binding, and necessitated a high-resolution fluorescence assay. As with oleic acid, smaller diameters of nAu-cap tended to impede ibuprofen binding, but no significance could be established.
Nanotechnology is a field of science and technology which is generally interested in particles that are smaller than 1-100 nm (billionth of a meter) and it is grown rapidly in recent years. It deals with molecules, atoms and these kind of structures which has nano-scale dimensions. Apart from macrosize materials, nanoscale structures have been shown novel and advanced features, thus they have attracted in especially electronic, textile, computer and pharmaceutical industries. Nanotechnology applications in the food industry have been developing more slowly than other areas because of their sensitivity and complex structures. However, in recent years, the food industry has done great progress in many areas such as the improvement of new packaging products, the development of new functional products, transport and controlled release of bioactive substances, detecting of pathogens by using nanosensors and indicators, and purification of water through the use of nanoparticles. In this article, the applications of nanotechnology in the food field and their status in the sector and the expectations of consumers are involved.
In Vivo Biocompatibility Evaluation of Gelatin–Hydroxyapatite Crosslink Biomimetic Scaffolds for Bone Regeneration
Biomimetic gelatin (gel)/hydroxyapatite (HA) scaffolds have been prepared for bone regeneration and hard tissue engineering. (A) 2.5% gel solely and (B) coprecipitation of 2.5% gel with 2.5% HA (w/w) were prepared to evaluate their biocompatibility, biodegradation and defect shape maintenance for regeneration. Sixteen week-old Vista rats (n=30) were used and calvarial defects were grafted with these scaffolds.
Investigation of Polydopamine Growth on Nanomaterials in Water
Capillary electrophoresis (CE) is a highly efficient separation technique useful for the analysis of nanomaterials in water. One limitation is the low sensitivity of detectors available for their quantification when mass spectrometry is not suitable. A new method based on polydopamine (PDA) growth was developed in our lab towards trace analysis of nanomaterials in aqueous suspension, using colloidal silica (SiO2) as a model inorganic oxide.