Assessment of Subacute Toxicity of Combined Exposure to Methyl morphine and Flunitrazepam Using a Rat Model

The aim of this study is to assess the toxic effects of combined abuse of these drugs. Methods: Thirty-five (35) male Wistar rats were divided into five (5) groups of seven animals each. Group 1 served as control and was administered distilled water only, groups 2 and 3 received a daily dose of 3.25 mg kg-1 bw of codeine and`0.03 mg kg-1 bw rohypnol respectively. Groups 4 and 5 both received combined doses of 3.25 mg kg-1 bw of codeine and 0.03mg kg-1 bw of rohypnol daily. All groups were exposed for 28 days except group 5 which was sacrificed after one week of withdrawal of treatments (on day 35). At the end of the exposure, some liver and brain function biomarkers were studied spectrophotometrically.