Comparison of Hemostatic Oppel and Cold Plasma Device for Partial Hepatectomy
Purpose: The main difficulty with liver surgery is blood loss. It is hard in this case to achieve hemostasis because of the oozing bleeding. The aim of this study was to compare the coagulation efficiency of cold plasma treatment with hemostatic Oppel during liver resection.
Methods: Adult male Wistar rats were randomly separated into 3 groups of 10 rats each. Group 1(control) included intact animals without surgical intervention; group 2 experimental)- animals after atypical liver resection with hemostatic suturing; group 3 (experimental)-animals after atypical liver resection with hemostasis using cold plasma. Histological and biochemical analysis were carried out on the 90th and 180th days after liver resection.
Results: Biochemical blood analysis revealed insignificant fluctuations in glucose (p<0.05) in experimental groups relative to the control group other parameters remained unchanged. Complete restoration of the organ structure was noted in the cold plasma group on the 180th day. Destructive changes have been preserved in the hemostatic Oppel group by this time.
Conclusions: Hemostasis with a cold plasma leads to a rapid stop of bleeding. In this case there is a recovery of all liver functions and of internal blood flow with the preservation of all biochemical blood parameters on the 180th day. Application of the hemostatic Oppel leads to dystrophic and necrotic changes in hepatocytes, as well as the morphological features of microvascular thrombosis and perivascular fibrosis which persist up to 180th day.