Journal of Pharmaceutical Sciences & Emerging Drugs .ISSN: 2380-9477

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Development of Topical Oral Anti-Inflammatory Gel Containing Zanthoxylum limonella seed Extracted: A Preclinical Study

Background: This study aims to investigate the stability of Zanthoxylum limonella Alston. seed (ZL) at various storage temperatures and anti-inflammatory activity of the topical oral gel (ZG) in a preclinical study.

Methods: The seed was stored in 2 different conditions: accelerated condition (50 °C, 70 °C and 90 °C) over 96 h and long-term condition (4 °C, 25 °C and 50 °C) over 6 months. Anti-inflammatory activity of the topical oral in rats was observed by using croton oil ear edema and oral punch wound healing.

Results: High storage temperature decreased the total phenolic content of ZL extract which the rate of degradation followed zero-order kinetic. In the long term condition, ZL stored at 50°C generated free radical while at 4 °C and 25 °C found the non-significant difference from the initial. The rate of release and permeation of phenolic content detected in receptor medium of the ZL into a mucoadhesive gel was 0.11 and 0.47 µg/cm2/h with no lag time indicated the fast release and permeation. The anti-inflammatory study suggests an effective dose of 10% of ZG which accelerated the wound size and reduced erythema compared to blank gel (p<0.001) and similar to FG (p=0.8) since the third day of treatment. The anti-inflammatory and wound healing activity of the ZG demonstrated re-epithelialized and wound closure, as supported by results of histopathology.

Conclusion: The topical oral gel contained the Zanthoxylum limonella seed extracts in the preclinical of rats was proved usefulness as an alternative topical oral product.

Special Features

Full Text

View

Track Your Manuscript

Recommended Conferences

Share This Page

Media Partners

Associations