Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Journal of Forensic Toxicology & PharmacologyISSN: 2325-9841
Alagandula R Author
Subjects of specialization
Affiliation
Quantification; Ethyl glucuronide; Urine; Flow injection; Mmultiple reaction monitoring
Department of Chemistry, Cleveland State University, 2121 Euclid Avenue, Cleveland, Ohio 44115, USA
Biography
| Alagandula R Department of Chemistry, Cleveland State University, 2121 Euclid Avenue, Cleveland, Ohio 44115, USA |
Publications
Research Article Open Access
A Rapid Dilute and Shoot - Flow Injection MRM Method for the Quantification of EtG in Urine
Author(s): Alagandula R and Guo B
Quantification of ethyl glucuronide (EtG, a metabolite and biomarker of ethanol) in urine is of great interest in both forensic and clinical applications. Currently, a single run of LC-MS/MS can quantify the vast majority of drugs and abused substances included in the comprehensive urine drug testing (UDT) panel, but EtG must be quantified in a separate run because EtG is polar, limiting the throughput of current MS-based methods. In this study, we developed a simple and fast MRM method to quantify EtG in urine without LC separation. Briefly, a urine sample containing EtG is first diluted 20 times, followed by direct flow-injection of the diluted sample to the ESI source. Quantification of EtG was achieved by multiple reaction monitoring (MRM) operated in th... view more»
Google Scholars / Researchers Sites
