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Hindi Advanced Biomedical Research and Innovation
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Jafar Ai Author
Subjects of specialization
Affiliation
Adolescent and Pediatric Gynecology, Anorexia Nervosa, Biochemistry of Reproductive Science, Biodiversity Management, Biodiversity and Conservation
Tehran University of Medical Sciences, Tehran, Iran
Biography
Jafar Aiwas was from Tehran University of Medical Sciences, Tehran, Iran ,Postdoctoral fellowship Tissue Engineering, University of Toronto, Toronto, Canada, Schooling form Advanced Technologies in Medicine Tissue Engineering
Publications
Short Article Open Access
Generation of artificial pancreas by using MIN-6 cell line culture seeded in decellularized testis's rat scaffold
Author(s): Samaneh Karimi, Jafar Ai, Layasadat Khorsandi , Azin samimi and Afrooz karimi
Objective: Type 1 diabetes is an autoimmune disease which is due to the lack of 𛽠cells. Regenerative medicine offers the opportunity for curing diabetes. Native extracellular matrix (ECM) creates a reconstruction platform to replace the organs. A decellularized rats testis was used as a natural 3D scaffold in this experiment. MIN_6 𛽠cells were planted in the scaffold. Methods: The decellularized testes were prepared for in vitro analyses. Results: The decellulalarazed testes were analyzed by histological studies such as H&E and DAPI staining. SEM showed that the 3D ultra structure of the testis remained intact. In vitro studies point out that the decellularized scaffold was non_toxic and preformed a good plateform for MIN_6 𛽠cells. The DTZ staining showed c... view more»
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