Padet Siriyasatien Author

Research Article Open Access

Profile of The Internal Transcribed Spacer 2 in Ribosomal DNA of Tabanus rubidus in Thailand and Myanmar; The Possibility of Using for Molecular Identification

Author(s):

Apiwat Tawatsin, Payu Bhakdeenuan, Yang Chao , Tomoko Amano , Hitoshi Sasaki, Usavadee Thavara, Somchai Sangkitporn, Theeraphap Chareonviriyaphap and Padet Siriyasatien*

Tabanus rubidus is one of the most medically and veterinary important haematophagous insects. We demonstrated the profile of the Internal Transcribed Spacer 2 (ITS2) of ribosomal DNA in T. rubidus collected from various regions of Thailand and Myanmar. The ITS2 sequences in this study is high in A+T content (80.1%) and ranged from 589 to 622 bp. The highest intra and interspecific divergence among samples from same region was 1.2% and 11.2%, respectively. The sequences from this study were grouped by the same collecting region into individual clade using the Neighbor Joining with Kimura 2-Parameter tree. The data obtained in this study is useful for accurate species identification using ITS2 sequence which is the first step to provide the b... view more»

DOI: 10.4172/2473-4810.1000121

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