Valdir de Queiroz Balbino Author

Research Article Open Access

Genetic Structure of Sympatric Populations of Female Lutzomyia Longipalpis (Diptera: Psychodidae) In Sobral and Caririaçu, Ceará State, Brazil

Author(s):

Moises Thiago de Souza Freitas*, César Raimundo Lima Costa Jr, Lidiane Gomes da Silva, Tereza Cristina Leal- Balbino, Reginaldo Peçanha Brazil and Valdir de Queiroz Balbino

Objectives: Accurate identification of these insects is critical to avoiding mistakes in the recognition of vector and non-vector species, so the invariant phenotypic patterns displayed by the females of L. longipalpis require the implementation of molecular methods of identification. Our goal was to examine genetic variation in the females of L. longipalpis using the SNPs reported in the males of this vector species.

Methods: We trapped sand flies near houses and domestic animal shelters at two localities in the state of Ceará, Brazil, using two to five CDC miniature light traps. Genomic DNA was separately extracted from each female using 100 μl of Chelex resin, and a... view more»

DOI: 10.4172/2473-4810.1000125

Abstract HTML PDF