Ventola A Author

Research Article Open Access

Multiple Dye Doped Core-Shell Silica Nanoparticles: Outstanding Stability and Signal Intensity Exploiting FRET Phenomenon for Biomedical Applications

Author(s):

Pellegrino C, Volpe A, Juris R, Menna M, Calabrese V, Sola F, Barattini C and Ventola A

We present a one-pot synthesis of core-shell silica nanoparticles (SiNPs) as a novel fluorescent probe for biological applications. SiNPs were doped with a different number of dyes to ensure high efficiency Fluorescence Resonance Energy Transfer (FRET). Dyes are individually entrapped in the silica core without a covalent bonding between them. The strong interconnection achieved inside the core, a FRET with efficiency up to 86% was obtained. Nanoparticles called NTB530, NTB575 and NTB660 contain two, three and four different dyes respectively. Nanoparticles can be excited with a common blue laser and characterized by a long Stokes Shift up to the near-IR emission. Photostability, tested under continuous irradiation with a mercury lamp, showed higher stabilit... view more»

DOI: 10.4172/2324-8777.S6-003

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