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Hindi Journal of Nanomaterials & Molecular NanotechnologyISSN: 2324-8777
Zlatina Becheva Author
Subjects of specialization
Affiliation
Flow Cytometry, Gel Electrophoresis, Affinity Chromatography, Immunoassay, Immunofluorescence
Department of Biotechnology, University
Biography
Zlatina Becheva working in the Department of Biotechnology, University “Prof. Dr Asen Zlatarov”, Bourgas, Bulgaria. Zlatina Becheva has an area of research interest in the field of Flow Cytometry, Gel Electrophoresis, Affinity Chromatography, Immunoassay, Immunofluorescence
Publications
Research Article Subscription
Preparation of Anti-Elastase Antibody Conjugated With Quantum Dots 710 Nm and Fluorescein Isothiocyanate for Immunoassay of Milk Somatic Cells
Author(s): Zlatina Becheva and Tzonka Godjevargova
Preparation of Anti-Elastase Antibody Conjugated With Quantum Dots 710 Nm and Fluorescein Isothiocyanate for Immunoassay of Milk Somatic Cells
A novel immunofluorescence microscopic method for a neutrophil and somatic cell count was developed by using anti-elastase antibody. Two different conjugates - anti-bovine neutrophil elastase antibody-fluorescein isothiocyanate conjugate and anti-bovine neutrophil elastase antibody-Quantum Dots 710nm conjugate were prepared. The coupling of Quantum Dots (QDs) and fluorescein isothiocyanate (FITC) to antibody has been demonstrated by comparison of ultraviolet absorption spectra and fluorescence spectra of conjugate and initial components. The activities of conjugates were measured by indirect E... view more»
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