Short Communication, Jbrhd Vol: 6 Issue: 6

Effect of a Three-Dimensional Matrix with Calcium Phosphate Coating on the Functional Activity of Jurkat Cells during Cultivation with MMSC

Khaziakhmatova Olga

Immanuel Kant Baltic Federal University, Russia

Abstract

Introduction: Currently, traumatology actively uses ceramic materials with calcium phosphate coating (CP). Such materials have a stimulating effect on multipotent mesenchymal stromal cells (MMSCs) and immunocompetent cells. So, their use involves an assessment of the impact of these materials on the development of tumor cells.

Methodology: The Jurkat 5332 cell line and human adipose-derived MMSCs (AMMSCs) were examined. 3D culture was simulated by adding to the cell culture the substrates from commercially pure titanium with rough (Ra=2-5 µm) CP microarc coating. The cultures were used: 2D with Jurkat T cells (JTCs) on plastic surface; 2D coculture of JTCs and AMMSCs on plastics; 3D with JTCs and CP matrix; 3D with JTCs, AMMSCs, and CP matrix.