Journal of Blood Research & Hematologic Diseases

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Short Communication, Jbrhd Vol: 6 Issue: 6

Effect of a Three-Dimensional Matrix with Calcium Phosphate Coating on the Functional Activity of Jurkat Cells during Cultivation with MMSC

Khaziakhmatova Olga

Immanuel Kant Baltic Federal University, Russia

Abstract

Introduction: Currently, traumatology actively uses ceramic materials with calcium phosphate coating (CP). Such materials have a stimulating effect on multipotent mesenchymal stromal cells (MMSCs) and immunocompetent cells. So, their use involves an assessment of the impact of these materials on the development of tumor cells.

 

Keywords:

Introduction: Currently, traumatology actively uses ceramic materials with calcium phosphate coating (CP). Such materials have a stimulating effect on multipotent mesenchymal stromal cells (MMSCs) and immunocompetent cells. So, their use involves an assessment of the impact of these materials on the development of tumor cells.

Methodology: The Jurkat 5332 cell line and human adipose-derived MMSCs (AMMSCs) were examined. 3D culture was simulated by adding to the cell culture the substrates from commercially pure titanium with rough (Ra=2-5 µm) CP microarc coating. The cultures were used: 2D with Jurkat T cells (JTCs) on plastic surface; 2D coculture of JTCs and AMMSCs on plastics; 3D with JTCs and CP matrix; 3D with JTCs, AMMSCs, and CP matrix.

Track Your Manuscript