Journal of Immunological Techniques & Infectious Diseases ISSN: 2329-9541

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Research Article, J Immunol Tech Infect Dis Vol: 5 Issue: 3

Epitope Mapping of a Long Peptide Candidate for Diagnosis of Hepatitis C - Evaluation of the Antigenicity by the Multiple Antigen Blot Assay (MABA)

Henry B1*, Adriana G1, Marilyan T2, Angelita LM1, Sandra L1, Daniel AL1, Nahir M3, Pierina DA4, Doneyla S4 and Oscar N1,2,5
1Biohelmintiasis section, Central Institute of Tropical Medicine "Dr. Felix Pifano", Faculty of Medicine, University of Venezuela, Venezuela
2Department of Parasitology, School of Medicine "Luis Razetti", Faculty of Medicine, Central University of Venezuela, Venezuela
3Section of Virology, Central Institute of Tropical Medicine, Faculty of Medicine, University of Venezuela, Venezuela
4Special laboratory - Hepatitis and AIDS Programs , National Institute of Hygiene "Rafael Rangel", Ministry of Popular Power for Health, Venezuela
5Malaria Center for Studies, Institute of Higher Studies "Dr. Arnoldo Gabaldon" - National Institute of Hygiene "Rafael Rangel", Ministry of Popular Power for Health, Caracas, Venezuela
Corresponding author : Henry Bermúdez
Biohelmintiasis section, Central Institute of Tropical Medicine "Dr. Felix Pifano", Faculty of Medicine, University of Venezuela, Venezuela
Tel: +58 416 9056723,+58 416 6389110
E-mail: [email protected]
Received: May 12, 2016 Accepted: June 27, 2016 Published: July 05, 2016
Citation: Henry B, Adriana G, Marilyan T, Angelita LM, Sandra L, et al. (2016) Epitope Mapping of a Long Peptide Candidate for Diagnosis of Hepatitis C -Evaluation of the Antigenicity by the Multiple Antigen Blot Assay (MABA). J Immunol Tech Infect Dis 5:3. doi:10.4172/2329-9541.1000147

Abstract

Epitope Mapping of a Long Peptide Candidate for Diagnosis of Hepatitis C - Evaluation of the Antigenicity by the Multiple Antigen Blot Assay (MABA)

Hepatitis C is a worldwide public health problem by its high prevalence and relevance and the impact in the high incidence of liver cirrhosis and liver cancer. Previous studies identified the peptide IMT-286 (40 mer), located in the core of the virus as a possible candidate to be used in the diagnosis of Hepatitis C by ELISA. The synthesis of long peptides in addition to its higher cost may be limited by undesirable reactions (cycling, deletions, etc.) that impede the synthesis. Therefore, we decided to reduce its size by selecting the potential epitopes of this sequence, whether corresponded to continuous or discontinuous primary sequences. For this, different peptide sequences were synthesized and evaluated for their antigenicity (SPOT mapping) by the pep scan strategy. Two peptides of Core antigen showed the highest sensitivities, peptide IMT-1700 (26 mer), located in the central region, presented a 59.09% recognition by sera from infected patients by the Multiple Antigen Blot Assay (MABA), while the IMT-286 (40 mer) peptide showed a recognition of 70.45 % The alkaline phosphatase conjugate showed a better sensitivity than the peroxidase conjugate with Luminol® as a substrate by MABA.

Keywords: Virus; Hepatitis C; Pep scan-SPOT; Peptides; MABA; Immunodiagnostic

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