Journal of Immunological Techniques & Infectious Diseases ISSN: 2329-9541

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Research Article, J Immunol Tech Infect Dis Vol: 5 Issue: 2

Rv3881c from Mycobacterium tuberculosis Elicits PolyFunctional CD8+ T cells in PPD Positive Healthy Volunteers and Affords Significant Protection in the Guinea Pig Model

Satchidanandam V1*, Kumar N1, Biswas S1, Jumani RS1, Jain C1, Rani R2, Aggarwal B2, Singh J2, Kotnur MR3, Challu V4, Chadha VK4, Kumar P4 and Sridharan A4
1Department of Microbiology and Cell Biology, Indian Institute of Science, Sir C.V. Raman Avenue, Bangalore, Karnataka 560012, India
2Molecular Immunogenetics Group, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India
3Department of Chest Medicine, MS Ramiah hospital, New BEL Road, Bangalore Karnataka 560054, India
4National Tuberculosis Institute, 8, Bellary Road, Bangalore, Karnataka 560003, India
Corresponding author : Vijaya Satchidanandam
Department of Microbiology and Cell Biology, Indian Institute of Science, Sir CV Raman Avenue, Bangalore, Karnataka 560012, India
Tel: 91-80-22932685
Fax: 91-80-23602697
E-mail: [email protected]
Received: October 29, 2015 Accepted: March 04, 2016 Published: March 10, 2016
Citation: Satchidanandam V, Kumar N, Biswas S, Jumani RS, Jain C, et al. (2016) Rv3881c from Mycobacterium tuberculosis Elicits Poly-Functional CD8+ T cells in PPD-Positive Healthy Volunteers and Affords Significant Protection in the Guinea Pig Model. J Immunol Tech Infect Dis 5:2. doi:10.4172/2329-9541.1000138

Abstract

We show that Rv3881c, a secreted protein of M. tuberculosis (MTB), elicits T cell responses from healthy PPD-positive individuals and TB patients. Synthetic peptides spanning the complete length of Rv3881c induced secretion of significantly greater levels of TNF-α and IL-10 from peripheral blood mononuclear cells of TB patients compared to latently infect healthy volunteers. Polychromatic flow cytometry detected poly-functional CD4+ and CD8+ T cells secreting different combinations of IFN-γ, TNF-α, IL-2 and MIP-1β in response to Rv3881c. Levels of CD4+ T cells secreting either IFN-γ or TNF-α were significantly higher in TB patients while levels of CD8+ T cells secreting both IFN-γ and IL-2 were significantly greater in healthy PPD-positive volunteers.

Keywords: Mycobacterium tuberculosis; Rv3881c; Human polyfunctional T cells; Guinea pig model; Experimental vaccine

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