Journal of Immunological Techniques & Infectious Diseases ISSN: 2329-9541

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Research Article, J Immunol Tech Infect Dis Vol: 5 Issue: 1

Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens

Perraut R1*, Varela M-L1, Mbengue B2, Guillotte M3, Mercereau-Puijalon O3 and Vigan-Womas I3
1Institute Pasteur of Dakar, Immunology Unit, Dakar, Senegal
2Institute Pasteur de Dakar, Immunology Unit, Dakar, Senegal
3Institute Pasteur, Molecular Immunology Unit of Parasite, 25-28 Rue du Dr Roux, 75015 Paris, France
Corresponding author : Ronald Perraut
Institute Pasteur de Dakar, Immunology Unit, Dakar, Senegal
E-mail: [email protected]
Received: October 01, 2015 Accepted: December 08, 2015 Published: December 14, 2015
Citation: Perraut R, Varela M-L, Mbengue B, Guillotte M, Mercereau-Puijalon O, et al. (2016) Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens. J Immunol Tech Infect Dis 5:1. doi:10.4172/2329-9541.1000134

Abstract

Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens

Multiplex assays are currently used to facilitate the evaluation of antibody (Ab) responses to multiple Plasmodium falciparum antigens from large field-based epidemiological studies. The present study aimed at (i) optimizing parameters of a novel cost-effective, compact and reliable magnetic bead-based multiplex immunoassay (MBA) carried out with the MAGPIX®-Luminex system and (ii) comparing the results with those obtained using standard ELISA technology.

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