Characterization of silver nanomaterials derived from marine Streptomyces sp. Al-Dhabi-87 and its in vitro application against multidrug-resistant and extended-spectrum beta-lactamase clinical pathogens

<p>Abdul Kareem Mohammed Ghilan, Naif Abdullah Al-Dhabi and Mariadhas Valan Arasu</p>

Characterization of silver nanomaterials derived from marine Streptomyces sp. Al-Dhabi-87 and its in vitro application against multidrug-resistant and extended-spectrum beta-lactamase clinical pathogens

Abdul Kareem Mohammed Ghilan, Naif Abdullah Al-Dhabi and Mariadhas Valan Arasu

King Saud University, Saudi Arabia

: J Nanomater Mol Nanotechnol

Abstract

A novel antagonistic marine Streptomyces sp. Al-Dhabi-87 that was recovered from the Gulf region of Saudi Arabia was used to synthesize silver nanoparticles (NP) from the culture free extract. The produced NP was confirmed by UV-visible spectroscopy (UV-Vis), high-resolution scanning electron microscope (HRSEM), transmission electron microscope (TEM), Fourier-transform infrared spectroscopy (FTIR), energy dispersive spectroscopy (EDAX) and X-ray powder diffraction (XRD) and broth microdilution techniques were employed for the determination of minimum inhibitory concentrations (MIC) values. The synthesized NP was authenticated by alterations in color and wavelength scanning. HRSEM and TEM analysis confirmed that the size of the NP ranged from 10 to 17nm and that it was spherical in shape. In addition, the FTIR spectrum revealed a variation in the band values from 500 to 3300cm-1 respectively. Rietveld refinement analysis of the XRD data confirmed the size of the NP, which coincided with the results of the TEM analysis. In addition, the riveted refinement analysis supported the TEM data. The NP documented significant activity against the wound infection microbial strains, such as Enterococcus faecalis, Staphylococcus epidermidis and Staphylococcus aureus. Gram-negative bacteria, such as Pseudomonas aeruginosa, Klebsiella pneumonia and Escherichia coli revealed MIC values of 0.039, 0.078 and 0.152mg/mL, respectively. The promising activity of NP towards extended-spectrum beta-lactamases E. coli, drug-resistant Acinetobacter baumannii and multidrugresistant S. aureus (at 0.018, 0.039 and 0.039mg/mL, respectively) was advantageous. Overall, NP that was obtained from the novel Streptomyces sp. Al-Dhabi-87, with its promising antimicrobial activity towards the drug-resistant pathogens, would be useful for healing infectious diseases.