Cytotoxicity effect of Citrullus colocynthis on oral squamous cell carcinoma
Shahin Gavanji
Esfahan University, Iran
: Dent Health Curr Res
Abstract
Statement of the Problem: Oral squamous cell carcinoma is the sixth common cancer in the world. Up to now, many endeavors have been done to cure oral squamous such as surgery, radiotherapy and chemotherapy. The clinical effectiveness of chemotherapy is limited which is due to its side effects, toxicity and drug resistance. Traditional medicine is being used for treatment of some diseases like cancers. Hence, the aim of this study is to examine the cytotoxicity effect of Citrullus colocynthis on oral squamous cell carcinoma. Methodology & Theoretical Orientation: In this in vitro study, oral squamous cell carcinoma cells (KB) and normal rat fibroblast cells (L929) as control group were cultured in enriched RPMI 1640 medium. Then extract of C. colocynthis was procured from the Iranian Research Center for Herbal and Traditional Medicine and the cells were treated with 0.1-50 μg/mL concentration of the extract for 24, 48 and 72 hours. Thereafter, the viability of the cells was assessed by Methyl Toluidine Blue (MTT) method. Statistical analysis was performed with SPSS 20, using one-way Analysis of Variance (ANOVA). Findings: The half-maximal inhibitory concentration (IC50) of C. colocynthis extract for KB cells and L929 cells were 12.18 and 43.53 μg/ml respectively, demonstrating that higher concentration of C. colocynthis extract is necessary to reach IC50 concentration for L929 cells (about 3.57 times). Conclusion & Significance: This study showed that the alcoholic extract of C. colocynthis exerted cytotoxic effects on tumoral cells of oral squamous cell carcinoma. Moreover, it exerted more cytotoxic effects on tumoral cells compared to normal cells.
Biography
Shahin Gavanji was selected as the Best Science and Technology Young by Ministry of Science, Research and Technology of Iran in 2013. He also could win the best young symbol from Iran president hands prize in 2010 for the second time. He was chosen as the best inventor of Iran in 2009.
E-mail: shahin.gavanji12@gmail.com
