Toxicogenomic analysis of the ability of brominated flame retardants TBBPA and BDE-209 to disrupt thyroid hormone signaling in neural cells
Frédéric Flamant and Romain Guyot
Université de Lyon, France
: Forensic Toxicol Pharmacol 2015, 4:3
Abstract
Brominated flame retardants are suspected to act as disruptors of thyroid hormone (T3) signaling. As T3 is well-known to be required for proper brain development, this raises the concern that brominated flame retardants might affect children’s cognitive functions. We performed an in vitro analysis of the ability of the most common compounds, tetrabromobisphenol A (TBBPA) and BDE-209, to alter thyroid hormone response based on a model neural cell line and genome-wide analysis of gene expression (RNAseq). We observed a modest but specific alteration of T3 signaling with both compounds. This study mainly illustrates the statistical power provided by genome wide transcriptome analysis in this cellular system to detect modest disruption in T3 signaling and isolate it from other signaling components. Our current effort to transpose this strategy to living mice will be presented.
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