Journal of Forensic Toxicology & PharmacologyISSN: 2325-9841

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Research Article, J Forensic Toxicol Pharmacol Vol: 4 Issue: 1

Elucidation of Acrylamide Genotoxicity and Neurotoxicty and the Protective Role of Gallic Acid and Green Tea

Abdelaziz E Ibrahim1*, Rania A El Kareem2 and Marwa A Sheir3
1Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
2Department of Histology and Histochemistry, Faculty of science, Suez University, Egypt
3Food Technology Research Institute, Agricultural Research Center, Giza, Egypt
Corresponding author : Abdelaziz E Ibrahim
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
E-mail: [email protected]
Received: February 27, 2015 Accepted: March 11, 2015 Published: March 13, 2015
Citation: Ibrahim AE, Kareem RAE, Sheir MA (2015) Elucidation of Acrylamide Genotoxicity and Neurotoxicity and the Protective Role of Gallic Acid and Green Tea. J Forensic Toxicol Pharmacol 4:1. doi:10.4172/2325-9841.1000135

Abstract

 Elucidation of Acrylamide Genotoxicity and Neurotoxicty and the Protective Role of Gallic Acid and Green Tea

Objective : Acrylamide is a chemical compound used in many technological applications and can be formed naturally when foods, especially those are rich in sugars and low in protein cooked at high temperatures during (e.g., frying, grilling , baking or toasting ) . It has several harmful health effects including neurotoxicity, carcinogenicity, reproductive toxicity, genotoxicity, and mutagenicity. Humans have chronic contact with acrylamide through eating, e.g., fried potato chips and/or French fries; cereal products, including bread, breakfast cereals, cakes and biscuits; as well as, roasted coffee and probably also from smoking. This study was carried out to investigate the protective effects of gallic acid and green tea on brain damage induced by acrylamide in rats. Material and Methods: Biochemical analysis using antioxidant enzymes as a markers of toxicity , molecular study using comet assay indicating DNA degenerative effect and histopatological examination of brain tissue . Results: Acrylamide caused a significant decrease in serum butyryl cholinesterase , lactate dehydrogenase , glutathione-S-transferase , glutathione peroxidase , superoxide dismutase and catalase activities while malonaldehyde content was significantly increased, Comet assay revealed that acrylamide caused DNA degeneration in brain, While histopathological studies showed moderate to marked changes in brain tissue by acrylamide . Conclusion : All these findings greatly indicating the genotoxic and neurotoxic effect of acrylamide that improved by treatment with gallic acid or green tea.

Keywords: Acrylamide; Genotoxicity; Neurotoxicity; Protective role; Gallic Acid; Green tea; Rat

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