Journal of Forensic Toxicology & PharmacologyISSN: 2325-9841

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Research Article, J Forensic Toxicol Pharmacol Vol: 1 Issue: 1

Solid Phase Extraction and Analysis of THC and Metabolites from Whole Blood using a Novel Automated Procedure using Liquid Chromatography and Tandem Mass Spectrometry(LC-MS/MS)

Jeffery Hackett1* and Albert A. Elian2
1UCT Inc., 2731 Bartram Road, Bristol, PA 19007, USA
2Massachusetts State Police Crime Laboratory, 59 Horsepond Road Sudbury, MA 01776, USA
Corresponding author : Jeffery Hackett
United Chemical Technologies, USA
Tel: 215-781-9255; Fax: 215 785 1225
E-mail: [email protected]
Received: September 14, 2012 Accepted: October 31, 2012 Published: November 05, 2012
Citation: Hackett J, Elian AA (2012) Solid Phase Extraction and Analysis of THC and Metabolites from Whole Blood using a Novel Automated Procedure using Liquid Chromatography and Tandem Mass Spectrometry (LC-MS/MS) J Forensic Toxicol Pharmacol 1:1. doi:10.4172/2325-9841.1000101

 

Abstract

Solid Phase Extraction and Analysis of THC and Metabolites from Whole Blood using a Novel Automated Procedure using Liquid Chromatography and Tandem Mass Spectrometry (LC-MS/MS)

In this study, solid phase extraction (SPE) is described using a novel automated procedure to isolate tetrahydrocannabininol and its metabolites from whole blood samples. SPE was performed on an automated system after samples of whole blood were precipitated with acetonitrile. The system was programmed to condition the SPE columns with methylene chloride, methanol,de-ionized (DI) water, and aqueous phosphate buffer (0.1 M pH 7). The samples were loaded by the robot after which the SPE columns were washed with DI water, aqueous phosphate buffer and dried also by the same unit.

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